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MonoVista CRS

MonoVista

New!!! Confocal Raman Systems brochure - download now!  

MonoVista CRS Confocal Raman System

Confocal Raman Microscopy, or Micro-spectroscopy, is becoming an invaluable tool within Biophysics, Biology and Biochemistry as it can provide more detailed information on the structural composition on cells and biological systems than other more commonly used techniques, such as infra-red absorption spectroscopy.

Princeton Instruments has over forty years of experience providing high performance CCDs and spectrographs, two of the key components of a high-end Raman system, to the global research community. We have used our vast experience within this market to create a series of confocal Raman spectrometers that bear the hallmarks of quality and high performance.

The MonoVista series of Confocal Raman Systems integrates Acton's single-stage spectrographs with our extensive range of high-performance CCDs and Olympus Upright BX51 and Olympus Inverted IX71 microscopes, to provide a dedicated and flexible research tool. A high-stability base plate ensures long-term stability and repeatable measurements, while our extensive range of detectors ensures that the system has the best sensitivity and detection limits for your particular application.

The MonoVista series of confocal raman systems are suitable for a wide range of life-science applications, as confocal Raman spectroscopy can be used as a structural probe for any type of cell or biochemical reaction. There is a choice of integrated lasers or the systems can be integrated with existing laser systems and a six position filter wheel, allowing maximum flexibility. The modular design also permits easy reconfiguration for a variety of experiments.

Features and Benefits

  • Completely integrated modular systems provide instant results and easy configuration.
  • Integration with Olympus Upright and Inverted microscopes provides spectroscopic data with diffraction limited spatially resolved accuracy.
  • Rigid coupling ensures long-term stability and repeatable measurements.
  • Enhance flexibility with both macro and confocal micro sampling available in a single system.
  • Integrated lasers available providing a complete Raman solution.
  • The support of multiple lasers and full spectral coverage from the UV to NIR allows easy use of different excitation wavelengths.
  • Maximise signal acquisition with our wide range of detector options.
  • Imaging spectrographs used for maximum confocal resolution
  • Motorized z-focus, xyz Raman mapping and auto-focus available.
  • Heating and cooling stages for various temperature ranges.

Confocal Raman Microscopy can be used with practically any sample morphology and provides improved axial and spatial resolution over conventional microscopy. It can be used to highlight a specific portion of a molecule by changing the excitation wavelength and can be used to analyze unstable intermediate species.

In addition to these unique advantages, it is non-destructive, requires no special sample preparation and water bands are usually extremely small and subtractable. These numerous benefits allow researchers to perform extremely detailed analysis of cells in their natural state and allow monitoring of the chemistry within individual cells.

Spectral information can be obtained from specific points, as small as 1um, within a sample and spectra obtained from multiple spots can be collected into a three-dimensional data cube, where the x and y dimensions represent spatial co-ordinates and the z dimension stores the spectral information, or a four dimensional data cube that incudes three spatial dimensions plus a spectral dimension.

Useful information can be gleaned from the multidimensional data structures by slicing them along any of the spatial or spectral co-ordinates. Thus a comprehensive picture of a sample and its components can be generated from its Raman signatures.

raman image

Three-dimensional data cube of a solid microparticle obtained with a Princeton Instruments Raman system